Human Diacylglycerol kinase Alpha (DGKA) ELISA Kit

SKU: orb782631

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dgka(Diacylglycerol kinase alpha).

Research Area

Protein Biochemistry

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DGKA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DGKA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DGKA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DGKA in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.064 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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Dgka ELISA Kit, DAGK1 ELISA Kit, DAG kinase alpha ELISA Kit, DAGK ELISA Kit, DAGK1diacylglycerol kinase ELISA Kit, ELISA Kit

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Human Diacylglycerol kinase Alpha (DGKA) ELISA Kit

Quick Database Links

UniProt

P23743

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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