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Human DKK1 Protein, His Tag
SKU: orb1743317
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Description
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Key Properties
−| Source | E.coli |
|---|---|
| Expression System | HEK293 |
| Target | DKK1 |
| Reactivity | Human |
| Tag | C-6×His Tag |
| Molecular Weight | The protein has a predicted molecular mass of 26.6 kDa after removal of the signal peptide. The apparent molecular mass of DKK1-His is approximately 35-55 kDa due to glycosylation. |
| Expression Region | DKK1(Thr32-His266) 6×His tag |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Purity | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 2mM DTT, pH 7.4. |
| Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Disclaimer | For research use only |
Alternative Names
−DKK-1;SK
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Greater than 95% as determined by reducing SDS-PAGE.
Quick Database Links
Gene Symbol
DKK1
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
Human DKK1 Protein, His Tag (orb1743317)
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