Human Early Growth Response Protein 2 (EGR2) ELISA Kit

SKU: orb866905

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Description

Sandwich Human EGR2 ELISA Kit

Research Area

Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EGR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EGR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGR2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.05 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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KROX20 ELISA Kit, Early growth response 2 ELISA Kit, KROX-20, Drosophila, homolog (early growth response-2) ELISA Kit, CMT1D ELISA Kit, AT591 ELISA Kit

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Human Early Growth Response Protein 2 (EGR2) ELISA Kit

Quick Database Links

UniProt

P11161

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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