Human Epididymal Protein 4 (HE4) ELISA Kit

SKU: orb782375

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HE4(Epididymal protein 4).

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HE4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HE4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HE4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HE4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 ng/mL
Sensitivity	5.15 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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Epididymis Protein 4 ELISA Kit, dJ461P17.6 ELISA Kit, EDDM4 ELISA Kit, epididymal protein 4 ELISA Kit, Epididymal secretory protein E4 ELISA Kit, Epididymis specific whey acidic protein type four disulfide core ELISA Kit, HE 4 ELISA Kit, Major epididymis specific protein E4 ELISA Kit, Major epididymis-specific protein E4 ELISA Kit, MGC57529 ELISA Kit, Putative protease inhibitor WAP5 ELISA Kit, WAP 5 ELISA Kit, WAP domain containing protein HE4 ELISA Kit, WAP domain containing protein HE4 V4 ELISA Kit, WAP four disulfide core domain 2 ELISA Kit, WAP four disulfide core domain protein 2 ELISA Kit, WAP four-disulfide core domain protein 2 ELISA Kit, WAP5 ELISA Kit, WFDC 2 ELISA Kit, WFDC2 ELISA Kit, WFDC2_HUMAN ELISA Kit

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Human Epididymal Protein 4 (HE4) ELISA Kit

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UniProt

Q14508

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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