Human Factor Related Apoptosis Ligand (FASL) ELISA Kit

SKU: orb774882

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Factor Related Apoptosis Ligand(FASL).

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Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FASL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FASL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FASL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FASL in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	6.2 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD178 ELISA Kit, CD95L ELISA Kit, CD95-L ELISA Kit, FASLG ELISA Kit, FASLG ELISA Kit, APT1LG1 ELISA Kit, APT1-LG1 ELISA Kit, TNFSF6 ELISA Kit, Fas Antigen Ligand ELISA Kit, TNF Superfamily Member 6 ELISA Kit, Tumor Necrosis Factor(ligand)Superfamily,Member 6 ELISA Kit

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Human Factor Related Apoptosis Ligand (FASL) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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