Human Filamin A Alpha (FLNa) ELISA Kit

SKU: orb778380

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Filamin A Alpha(FLNa).

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 120 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FLNa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FLNa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FLNa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FLNa in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	1.88-120 ng/mL
Sensitivity	0.62 ng/mL
Concentration	120 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ABP280 ELISA Kit, FLN-A ELISA Kit, ABPX ELISA Kit, FLN ELISA Kit, FLN1 ELISA Kit, FMD ELISA Kit, MNS ELISA Kit, NHBP ELISA Kit, OPD ELISA Kit, OPD1 ELISA Kit, OPD2 ELISA Kit, Actin Binding Protein 280 ELISA Kit, Endothelial actin-binding protein ELISA Kit, Filamin-1 ELISA Kit, Non-muscle filamin ELISA Kit

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Quick Database Links

UniProt

P21333

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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