Human FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) ELISA Kit

SKU: orb778180

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FK506 Binding Protein 12 Rapamycin Associated Protein(FRAP).

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 20 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human FRAP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human FRAP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human FRAP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human FRAP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.32-20 ng/mL
Sensitivity	0.116 ng/mL
Concentration	20 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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FRAP1 ELISA Kit, MTOR ELISA Kit, FRAP2 ELISA Kit, MTOR ELISA Kit, RAFT1 ELISA Kit, RAPT1 ELISA Kit, Mammalian Target Of Rapamycin ELISA Kit, FKBP12-Rapamycin Complex-Associated Protein 1 ELISA Kit, Rapamycin and FKBP12 target 1 ELISA Kit

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Human FK506 Binding Protein 12 Rapamycin Associated Protein (FRAP) ELISA Kit

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UniProt

P42345

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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