Human GFRA2 protein

SKU: orb391796

Description

Recombinant human GFRA2 protein is produced by our Mammalian expression system and the target gene encoding Ser22-Ser441 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Reactivity	Human
Protein Sequence	SPSSLQGPEL HGWRPPVDCV RANELCAAES NCSSRYRTLR QCLAGRDRNT MLANKECQAA LEVLQESPLY DCRCKRGMKK ELQCLQIYWS IHLGLTEGEE FYEASPYEPV TSRLSDIFRL ASIFSGTGAD PVVSAKSNHC LDAAKACNLN DNCKKLRSSY ISICNREISP TERCNRRKCH KALRQFFDRV PSEYTYRMLF CSCQDQACAE RRRQTILPSC SYEDKEKPNC LDLRGVCRTD HLCRSRLADF HANCRASYQT VTSCPADNYQ ACLGSYAGMI GFDMTPNYVD SSPTGIVVSP WCSCRGSGNM EEECEKFLRD FTENPCLRNA IQAFGNGTDV NVSPKGPSFQ ATQAPRVEKT PSLPDDLSDS TSLGTSVITT CTSVQEQGLK ANNSKELSMC FTELTTNIIP GSNKVIKPNS VDHHHHHH
Purity	Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
Disclaimer	For research use only

Alternative Names

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GDNFRB; RETL2; TRNR2; GDNF family receptor alpha-2; GDNF receptor alpha-2; GDNFR-alpha-2; GFR-alpha-2; GDNF receptor beta; GDNFR-beta; Neurturin receptor alpha; NRTNR-alpha; NTNR-alpha; RET ligand 2; TGF-beta-related neurotrophic factor receptor 2

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Quick Database Links

UniProt

O00451

NCBI Gene ID

2675

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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