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Human HGF Protein, His Tag

SKU: orb700640
ActiveBiologically Active

Description

Human HGF, His Tag, premium grade is expressed from human 293 cells (HEK293). It contains AA Gln 32 - Ser 728 (Accession # P14210-1).

Research Area

Immunology & Inflammation

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. The mature form of HGF is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 81.6 kDa (α chain 53.7 kDa and β chain 27.9 kDa). The protein migrates as 80-90 kDa (alpha & Beta chain), 58-63 kDa (alpha chain), 33 kDa (Beta chain) when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
TargetHGF
ReactivityHuman
TagC-10×His
Molecular Weight81.6 kDa (53.7 kDa|27.9 kDa)
Expression RegionGln 32 - Ser 728
Purity90%
Endotoxins0.1 EU per μg

Storage & Handling

Storage-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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Human HGF Protein, His Tag

Human HGF R, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human HGF, His Tag, premium grade (Cat. No. orb700640) with an affinity constant of 0.486 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

UniProt Details

No UniProt data available

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Human HGF Protein, His Tag (orb700640)

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20 μg
¥ 4,940.00
100 μg
¥ 10,530.00
1 mg
¥ 44,330.00
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