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Human IGF-I R Protein, His Tag

SKU: orb511230
ActiveBiologically Active

Description

Human IGF-I R, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 31 - Asn 932 (Accession # P08069-1).

Research Area

Disease Biomarkers

Images & Validation

Application Notes
This protein carries a polyhistidine tag at the C-terminus. This protein contains a furin convertase cleavage site, 737-RKRR-740, and will be partially processed into N (α chain) and C-terminal fragment (partial β chain) with calculated MW of 81.0 kDa and 23.8 kDa respectively. The protein migrates as 35-45 kDa, 110-120 kDa and 130 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

Expression SystemHEK293
TargetIGF-I R / IGF-1 R
ReactivityHuman
TagC-10×His
Molecular Weight104.8 kDa
Expression RegionGlu 31 - Asn 932
Purity90%
Endotoxins1.0 EU per μg
ConjugationUnconjugated

Storage & Handling

Storage-20°C
Form/AppearancePowder
Buffer/PreservativesPBS, pH 7.4
Expiration Date6 months from date of receipt.
DisclaimerFor research use only

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Human IGF-I R Protein, His Tag

Human IGF-I R, His Tag (Cat. No. orb511230) immobilized on CM5 Chip can bind Biotinylated Human IGF-I, His, Avitag with an affinity constant of 68 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product
RefSeqP08069

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Protocol Information

Human IGF-I R Protein, His Tag (orb511230)

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100 μg
¥ 9,230.00
1 mg
¥ 57,850.00
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