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Human IL-10
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Activity | BLI: Loaded Human IL-10RA-His(Cat#orb2994251) on HIS1K Biosensor, can bind Human IL-10 (Cat#orb3002470) with an affinity constant of 1 pM as determined in BLI assay. (Regularly tested) |
| Tag | Tag free |
| Molecular Weight | Predicted: 18.6 KDa. Observed: 16 KDa, reducing conditions |
| Expression Region | Ser19-Asn178 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.. SEC-HPLC: Greater than 95% as determined by SEC-HPLC. (QC verified) |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS,4%Mannitol,pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
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Human IL-10 (orb3002470)
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