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Recombinant Human IL-1a Protein
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Tag | Tag free |
| Molecular Weight | Predicted: 18 KDa. Observed: 17 KDa, reducing conditions |
| Expression Region | Ser113-Ala271 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 7.5. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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−Recombinant human IL-1 Alpha protein, His (HEK293) [orb1516668]
>95% as determined by SDS-PAGE
25 kDa
100 μg, 500 μg, 20 μgHuman IL1A Protein, mFc Tag [orb2321373]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.3 kDa after removal of the signal peptide. The apparent molecular mass of IL1A-mFc is approximately 35-70 kDa due to glycosylation.
Mammalian
10 μg, 50 μg, 100 μgHuman IL1A Protein, hFc Tag [orb1173724]
Unconjugated
The purity of the protein is greater than 90% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.2 kDa after removal of the signal peptide.The apparent molecular mass of IL1A-hFc is approximately 35-55 kDa due to glycosylation.
Mammalian
50 μg, 10 μg, 100 μgRecombinant Human IL-1a Protein (Biotin) [orb3002217]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 20 KDa. Observed: 19 KDa, reducing conditions
20 μg, 100 μgRecombinant Human IL-1a Protein [orb3002226]
SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 44.9 KDa. Observed: 54 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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UniProt Details
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Protocol Information
Recombinant Human IL-1a Protein (orb3002450)
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