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Human IL-2
SKU: orb3002347
Description
Images & Validation
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Key Properties
−| Expression System | E.coli |
|---|---|
| Biological Activity | BLI: Loaded Human IL-2RA-His(Cat#orb2994464) on HIS1K Biosensor, can bind Human IL-2(Cat#orb3002347) with an affinity constant of 31 nM as determined in BLI assay. Loaded Human IL-2RA-Fc(Cat#orb2994463) on Protein A Biosensor, can bind Human IL-2(Cat#orb3002347) with an affinity constant of 13.2 nM as determined in BLI assay. Loaded Human IL-2RB-His(Cat#orb2994462) on HIS1K Biosensor, can bind Human IL-2(Cat#orb3002347) with an affinity constant of 0.19 uM as determined in BLI assay. Loaded Human IL-2RB-Fc(Cat#orb2994461) on Protein A Biosensor, can bind Human IL-2(Cat#orb3002347) with an affinity constant of 0.27 uM as determined in BLI assay. Loaded Mouse IL-2RA-His(Cat#orb2994413) on HIS1K Biosensor, can bind Human IL-2(Cat#orb3002347) with an affinity constant of 33 nM as determined in BLI assay. Loaded Biotinylated Human IL-2RB-His-Avi(Cat#orb2993868) on HIS1K Biosensor, can bind Human IL-2(Cat#orb3002347) with an affinity constant of 0.34 uM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. The ED50 for this effect is 90-270 pg/ml. |
| Tag | Tag free |
| Molecular Weight | Predicted: 15.5 KDa. Observed: 14 KDa, reducing conditions |
| Expression Region | Pro22-Thr153(Cys145Ser) |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 1 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 10mM Acetata-Na, 5% Trehaiose, pH 4.5. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-2; IL-2; T-cell growth factor; TCGF; Aldesleukin
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Human IL-2 (orb3002347)
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