Human IL-2

SKU: orb3002442

Description

Recombinant Human Interleukin-2 is produced by our Mammalian expression system and the target gene encoding Ala21-Thr153 is expressed with a 6His tag at the C-terminus.

Images & Validation

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Key Properties

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Expression System	Human Cells
Biological Activity	BLI: Loaded Human IL-2RA-Fc(Cat#orb2994463) on Protein A Biosensor, can bind Human IL-2-His(Cat#orb3002442) with an affinity constant of 3.94 nM as determined in BLI assay. Loaded Human IL-2RB-Fc(Cat#orb2994461) on Protein A Biosensor, can bind Human IL-2-His(Cat#orb3002442) with an affinity constant of 0.17 uM as determined in BLI assay.. Cell Based Assay: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells The specific activity of Recombinant Human IL-2 is ≥1×107 IU/mg.
Tag	C-6His
Molecular Weight	Predicted: 16.4 KDa. Observed: 14-18 KDa, reducing conditions
Expression Region	Ala21-Thr153
Purity	SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. (QC verified)
Endotoxins	< 0.01 EU/µg as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of 10mM Sodium Citrate, 4% Mannitol, pH 4.0
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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Interleukin-2; IL-2; T-Cell Growth Factor; TCGF; Aldesleukin; IL2

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UniProt

P60568

UniProt Details

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No UniProt data available

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Human IL-2 (orb3002442)

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Applications:	Blocking, FC, IF, IHC, WB
Reactivity:	Human, Primate
Species/Host:	Human
Clonality:	Monoclonal
Conjugation:	Unconjugated

Applications:	Blocking, FC
Reactivity:	Human, Primate
Species/Host:	Human
Clonality:	Monoclonal
Conjugation:	Unconjugated

Reactivity:	Human
Dynamic Range:	7.82-500pg/mL
Sensitivity:	3.1 pg/mL

Reactivity:	Human
Dynamic Range:	7.82-500pg/mL
Sensitivity:	4.69 pg/mL

Reactivity:	Human
Dynamic Range:	31.25-2000 pg/mL
Sensitivity:	17 pg/mL

Human IL-2

Description

Images & Validation

Key Properties

Storage & Handling

Alternative Names

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Human IL-2 (orb3002442)

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