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Human IL-4
SKU: orb3002477
Description
Images & Validation
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Key Properties
−| Expression System | Human Cells |
|---|---|
| Biological Activity | BLI: Loaded Cynomolgus IL-4RA-His(Cat#orb2994860) on HIS1K Biosensor, can bind Human IL-4(Cat#orb3002477) with an affinity constant of 0.33 nM as determined in BLI assay. Loaded Cynomolgus IL-4RA-Fc(Cat#orb2993918) on Protein A Biosensor, can bind Human IL-4(Cat#orb3002477) with an affinity constant of 0.54 nM as determined in BLI assay. Loaded Human IL-4RA-His(Cat#orb2994200) on HIS1K Biosensor, can bind Human IL-4(Cat#orb3002477) with an affinity constant of 0.48 nM as determined in BLI assay. Loaded Human IL-4RA-Fc(Cat#orb2994196) on Protein A Biosensor, can bind Human IL-4(Cat#orb3002477) with an affinity constant of 0.99 nM as determined in BLI assay. . Cell Based Assay: Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.01-0.05 ng/ml. (QC verified) |
| Tag | Tag free |
| Molecular Weight | Predicted: 14.97 KDa. Observed: 18 KDa, reducing conditions |
| Expression Region | His25-Ser153 |
| Purity | SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | < 0.01 EU/µg as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Reconstitution | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4
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Human IL-4 (orb3002477)
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