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Human IL10 protein
SKU: orb391908
Description
Research Area
Epigenetics
Images & Validation
−
| Tested Applications | ELISA, MS, SDS-PAGE, WB |
|---|---|
| Application Notes |
Key Properties
−| Source | Human cells |
|---|---|
| Reactivity | Human |
| Protein Sequence | SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxins | Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage & Handling
−| Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
|---|---|
| Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Disclaimer | For research use only |
Alternative Names
−Interleukin-10; IL-10; Cytokine synthesis inhibitory factor; CSIF
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The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 44.8 kDa after removal of the signal peptide. The apparent molecular mass of hFc-IL10 is approximately 35-55 kDa due to glycosylation.
Mammalian
50 μg, 10 μg, 100 μg
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
SDS-PAGE
Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis
Human IL10 protein (orb391908)
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