IL1B/IL1F2 Mouse Monoclonal Antibody

SKU: orb2974735

Description

Anti-Human IL1B/IL1F2 Antibody (BUCC2580) is a mouse monoclonal antibody detecting Interleukin-1 beta in ELISA, FCM. Suitable for Human. Highlights Flow Cytometry: Validated for flow cytometry applications.

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, FC
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a, kappa
Clone No.	BUCC2580
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4, 0.09% Sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Interleukin-1 beta, IL1B, Catabolin, IL1F2, IL-1 beta, IL-1β

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Flow-cytometry using anti-human IL1B antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1B monoclonal antibody (Catalog orb2974735, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Quick Database Links

UniProt

P01584

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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