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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, FC |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Human |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG4, kappa |
| Clone No. | BUCC2514 |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin | Please contact with the lab for this information. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M PBS, pH 7.4, 0.09% Sodium azide. |
| Concentration | Lot dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Flow-cytometry using anti-human IL23A antibody. IL23A Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL23A antibody monoclonal antibody (Catalog # orb2974711, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Western blot analysis was performed using anti-IL23A monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human IL23A (Catalog No: orb2967278), Lane 2: negative control.
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Protocol Information
IL23A Human Monoclonal Antibody (orb2974711)
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