Human Inducible T-Cell Co Stimulator (ICOS) ELISA Kit

SKU: orb776998

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inducible T-Cell Co Stimulator(ICOS).

Research Area

Cancer Biology, Immunology & Inflammation, Infectious Disease & Virology, Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ICOS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ICOS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ICOS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ICOS in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, urine, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	5.6 pg/mL
Concentration	1000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD278 ELISA Kit, AILIM ELISA Kit, Inducible T-Cell Costimulator ELISA Kit, Activation-Inducible Lymphocyte Immunomediatory Molecule ELISA Kit

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Human Inducible T-Cell Co Stimulator (ICOS) ELISA Kit

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UniProt

Q9Y6W8

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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