Human Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit

SKU: orb775209

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interferon Inducible T-Cell Alpha Chemoattractant(ITaC).

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Tested Applications

ELISA

Application Notes

standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITaC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITaC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITaC, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITaC in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	62.5-4000 pg/mL
Sensitivity	23 pg/mL
Concentration	4000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CXCL11 ELISA Kit, I-TAC ELISA Kit, IP9 ELISA Kit, SCYB11 ELISA Kit, SCYB9B ELISA Kit, B-R1 ELISA Kit, Chemokine(C-X-C-Motif)ligand 11 ELISA Kit, Small Inducible Cytokine Subfamily B Member 11 ELISA Kit, Interferon gamma-inducible protein 9 ELISA Kit

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Human Interferon Inducible T-Cell Alpha Chemoattractant (ITaC) ELISA Kit

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UniProt

O14625

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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