Human Interleukin 27A (IL27A) ELISA Kit

SKU: orb2667089

Description

Images & Validation

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Application Notes

standard: 1000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL27A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL27A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL27A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL27A in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Sample Types	Serum, plasma, tissue homogenates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	15.63-1000 pg/mL
Sensitivity	6.32 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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IL-27; IL27A; IL30; IL-27A; p28; IL-27 subunit alpha; IL-27-A; IL27-A

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