Human Kynurenine (KYN) ELISA Kit

SKU: orb782820

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to KYN(Kynurenine).

Research Area

Metabolism Research

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 500 pmol/ml. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KYN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KYN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KYN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KYN in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, saliva, cell lysates, follicular fluid and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	7.82-500 pmol/mL
Sensitivity	3 pmol/mL
Concentration	500 pmol/ml

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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KYN ELISA Kit, Kynurenine ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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