Human LEFTY1 protein

SKU: orb707060

Description

Recombinant Human TGF beta 4 Protein is produced by E.coli expression system and the target gene encoding Met1-Ser155 is expressed.

Research Area

Developmental Biology, Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Reactivity	Human
Protein Sequence	MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLIAEERG VVSIKGVCAN RYLAMKEDGR LLASKSVTDE CFFFERLESN GYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized from a 0.2 μm filtered solution of 20mM Tris, 150mM NaCl, pH 7.5.
Disclaimer	For research use only

Alternative Names

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LEFTB; LEFTYB; Left-right determination factor 1; Left-right determination factor B; Protein lefty-1; Protein lefty-B

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Quick Database Links

UniProt

O75610

NCBI Gene ID

10637

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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