Human LEFTY2 protein

SKU: orb706999

Description

Recombinant Human LEFTY2 Protein is produced by mammalian expression system and the target gene encoding Phe78-Pro366 is expressed fused with a Mouse Lefty-1 Propeptide (Leu22-Arg77) & 6His tag at the N-terminus.

Research Area

Stem Cell & Developmental Biology

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Target	LEFTY2
Reactivity	Human
Protein Sequence	LTGEQILGSL LQQLQLDQPP VLDKADVEGM VIPSHVRTQY VALLQHSHAS RSRGKRHHHH HHFSQSFREV AGRFLASEAS THLLVFGMEQ RLPPNSELVQ AVLRLFQEPV PKAALHRHGR LSPRSAQARV TVEWLRVRDD GSNRTSLIDS RLVSVHESGW KAFDVTEAVN FWQQLSRPRQ PLLLQVSVQR EHLGPLASGA HKLVRFASQG APAGLGEPQL ELHTLDLRDY GAQGDCDPEA PMTEGTRCCR QEMYIDLQGM KWAKNWVLEP PGFLAYECVG TCQQPPEALA FNWPFLGPRQ CIASETASLP MIVSIKEGGR TRPQVVSLPN MRVQKCSCAS DGALVPRRLQ P
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Endotoxins	Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μm filtered solution of PBS, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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EBAF; LEFTA; LEFTYA; TGFB4; Left-right determination factor 2; Endometrial bleeding-associated factor; Left-right determination factor A; Protein lefty-2; Protein lefty-A; Transforming growth factor beta-4; TGF-beta-4

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Quick Database Links

Gene Symbol

LEFTY2

UniProt

O00292

NCBI Gene ID

7044

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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