Human Microtubule-Associated Proteins 1A/1B Light Chain 3B (MAP1LC3B) ELISA Kit

SKU: orb782414

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B).

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Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAP1LC3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAP1LC3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAP1LC3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAP1LC3B in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.057 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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LC3B ELISA Kit, MAP1LC3B ELISA Kit, MAP1ALC3 ELISA Kit, Microtubule-associated proteins 1A/1B light chain 3B ELISA Kit, Autophagy-related protein LC3 B ELISA Kit, Autophagy-related ubiquitin-like modifier LC3 B ELISA Kit, MAP1 light chain 3-like protein 2 ELISA Kit, MAP1A/MAP ELISA Kit

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Human Microtubule-Associated Proteins 1A/1B Light Chain 3B (MAP1LC3B) ELISA Kit

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UniProt

A6NCE7

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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