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Description
Images & Validation
−Item 1 of 4
| Tested Applications | Blocking, ELISA, FC, ICC, IF, WB |
|---|---|
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1, kappa |
| Clone No. | BNGOX4X37 |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein A/G purified from cell culture supernatant. |
| Endotoxin | Please contact with the lab for this information. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.01M PBS, pH 7.4. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−MT-MMP 1, Matrix metalloproteinase-14, Membrane-type matrix metalloproteinase 1, Membrane-type-1 matrix metalloproteinase, MMP14, MTMMP1, MT1-MMP, MMP-14, MMP-X1, MT1MMP
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Flow-cytometry using anti-human MMP14 antibody. BXPC3 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 monoclonal antibody (Catalog orb2960653, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.
Flow-cytometry using anti-human MMP14 antibody. MDA-MB-231 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human MMP14 antibody monoclonal antibody (Catalog # orb2960653, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human MMP14 Antibody.
Western blot analysis was performed using anti-MMP14 monoclonal antibody at 1 µg/mL on various samples. Lane 1: recombinant human MMP14 (Catalog No: orb2966075), Lane 2: negative control.
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UniProt Details
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
MMP14 Mouse Monoclonal Antibody (orb2960653)
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