Human Neprilysin (NEP) ELISA Kit

SKU: orb776060

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neprilysin(NEP).

Images & Validation

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Tested Applications

ELISA

Application Notes

standard: 2000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NEP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NEP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEP in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, cerebrospinal fluid and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	31.25-2000 pg/mL
Sensitivity	11.6 pg/mL
Concentration	2000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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CD10 ELISA Kit, MME ELISA Kit, CALLA ELISA Kit, SFE ELISA Kit, Atriopeptidase ELISA Kit, Enkephalinase ELISA Kit, Skin fibroblast elastase ELISA Kit, Common Acute Lymphoblastic Leukemia Antigen ELISA Kit, Membrane Metallo-Endopeptidase ELISA Kit

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UniProt

P08473

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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