Human Neprilysin Protein

SKU: orb257693

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Description

Human Neprilysin, Tag Free is expressed from human 293 cells (HEK293). It contains AA Tyr 52 - Trp 750 (Accession # NP_000893.2).

Images & Validation

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Application Notes	This protein carries no "tag". The protein has a calculated MW of 80.0 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Key Properties

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Expression System	HEK293
Target	Neprilysin
Reactivity	Human
Tag	Native
Molecular Weight	80.0 kDa
Expression Region	Tyr 52 - Trp 750
Purity	95%
Endotoxins	1.0 EU per μg
Conjugation	Unconjugated

Storage & Handling

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Storage	-20°C to -70°C for 12 months in lyophilized state;. -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower
Form/Appearance	Powder
Buffer/Preservatives	20 mM Tris, 100 mM NaCl, pH 8.0
Disclaimer	For research use only

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SDS-PAGE analysis of Human Neprilysin protein

Quick Database Links

Gene Symbol

Neprilysin

UniProt

NP_000893.2

UniProt Details

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No UniProt data available

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Protocol Information

Find More Protocols

Willard, Joshua R. et al. Improved glycaemia in high-fat-fed neprilysin-deficient mice is associated with reduced DPP-4 activity and increased active GLP-1 levels Diabetologia, 60, 701-708 (2017)

PubMed: 27933334