PCSK9 Human Monoclonal Antibody

SKU: orb2975388

Description

Anti-Human PCSK9 Antibody (BUCC2371) is a human monoclonal antibody detecting Proprotein convertase subtilisin/kexin type 9 in FCM. Suitable for Human. Highlights Flow Cytometry: Validated for flow cytometry applications.

Research Area

Metabolism Research

Images & Validation

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Item 1 of 3

Tested Applications	FC
Reactivity	Human

Key Properties

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Host	Human
Clonality	Monoclonal
Isotype	IgG4, kappa
Clone No.	BUCC2371
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4.
Concentration	Lot dependent
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Proprotein convertase subtilisin/kexin type 9, Subtilisin/kexin-like protease PC9, NARC-1, PC9, Proprotein convertase 9, Neural apoptosis-regulated convertase 1, NARC1, PCSK9

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Flow-cytometry using anti-human PCSK9 antibody. HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PCSK9 antibody monoclonal antibody (Catalog # orb2975388, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human PCSK9 antibody. Human Jurkat cell line were stained with an irrelevant antibody (Blue Histogram) or an anti-human PCSK9 antibody monoclonal antibody (Catalog # orb2975388, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human PCSK9 antibody. PCSK9 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PCSK9 antibody monoclonal antibody (Catalog # orb2975388, Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody and cells analysed on a NovoCyte Flow Cytometer.

Quick Database Links

UniProt

Q8NBP7

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No UniProt data available

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Protocol Information

Flow Cytometry

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