Human PD-L1 Protein, mFc-His tag

SKU: orb689384

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Description

Recombinant human PD-L1 protein with C-terminal mouse Fc and 6×His tag

Images & Validation

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Item 1 of 4

Key Properties

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Source	Mammalian
Target	PDL1
Reactivity	Human
Tag	C-Mouse Fc and 6×His Tag
Molecular Weight	The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
Purification	Purified from cell culture supernatant by affinity chromatography
Purity	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.

Storage & Handling

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Storage	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Form/Appearance	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Buffer/Preservatives	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Disclaimer	For research use only

Alternative Names

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PD-L1;CD274;B7-H1;PDCD1L1;PDCD1LG1

Quality Guarantee

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Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-1, hFc tagged protein orb689457 can bind Human CD28, mFc-His tagged protein (orb689375) in a linear range of 125-4000 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-2, hFc tagged protein orb689458 can bind Human CD28, mFc-His tagged protein (orb689375) in a linear range of 62.5-4000 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28, mFc-His tagged protein (orb689375) 1 μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Quick Database Links

Gene Symbol

PDL1

UniProt

Q9NZQ7

UniProt Details

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No UniProt data available

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