Human PD-L1 Protein, mFc-His tag

SKU: orb689384

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Description

Recombinant human PD-L1 protein with C-terminal mouse Fc and 6×His tag

Research Area

Epigenetics, Immunology

Images & Validation

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Item 1 of 4

Key Properties

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Source	Mammalian
Expression System	HEK293
Target	PDL1
Reactivity	Human
Tag	C-Mouse Fc and 6×His Tag
Molecular Weight	The protein has a predicted molecular mass of 52.3 kDa after removal of the signal peptide.
Expression Region	PD-L1(Phe19-Arg238) mFc(Pro99-Lys330) 6×His tag
Purification	Purified from cell culture supernatant by affinity chromatography
Purity	The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Conjugation	Unconjugated

Storage & Handling

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Storage	Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Form/Appearance	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Buffer/Preservatives	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Reconstitution	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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PD-L1;CD274;B7-H1;PDCD1L1;PDCD1LG1

Quality Guarantee

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Human CD28 Protein, mFc-His Tag on SDS-PAGE under reducing condition.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-1, hFc tagged protein orb689457 can bind Human CD28, mFc-His tagged protein (orb689375) in a linear range of 125-4000 ng/ml.

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human B7-2, hFc tagged protein orb689458 can bind Human CD28, mFc-His tagged protein (orb689375) in a linear range of 62.5-4000 ng/ml.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28, mFc-His tagged protein (orb689375) 1 μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Quick Database Links

Gene Symbol

PDL1

UniProt

Q9NZQ7

UniProt Details

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No UniProt data available

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