Human Retinol Binding Protein 4, Plasma (RBP4) ELISA Kit

SKU: orb2668108

Description

Research Area

Metabolism Research

Images & Validation

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Application Notes

standard: 150 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human RBP4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human RBP4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human RBP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human RBP4 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	2.34-150 ng/mL
Sensitivity	0.69 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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PRBP; RBP; Plasma retinol-binding protein

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Quick Database Links

UniProt

P02753

UniProt Details

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No UniProt data available

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