Human S-Phase Kinase Associated Protein 2 (SKP2) ELISA Kit

SKU: orb778449

Featured

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to S-Phase Kinase Associated Protein 2(SKP2).

Images & Validation

−

Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SKP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SKP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SKP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SKP2 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

−

Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids.
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.062 ng/mL
Concentration	10 ng/mL

Storage & Handling

−

Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

−

FBL1 ELISA Kit, FBXL1 ELISA Kit, FLB1 ELISA Kit, p45 ELISA Kit, Cyclin-A/CDK2-associated protein p45 ELISA Kit, F-box/LRR-repeat protein 1 ELISA Kit, p45skp2 ELISA Kit

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Human S-Phase Kinase Associated Protein 2 (SKP2) ELISA Kit

Quick Database Links

UniProt

Q13309

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Manual

Product Manual

Download

Request a Document

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Find More Protocols