Human Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit

SKU: orb775740

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Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Secretory Leukocyte Peptidase Inhibitor(SLPI).

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Tested Applications

ELISA

Application Notes

standard: 4000 pg/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SLPI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SLPI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SLPI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SLPI in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Serum, plasma and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	62.5-4000 pg/mL
Sensitivity	27.5 pg/mL
Concentration	4000 pg/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Disclaimer	For research use only

Alternative Names

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ALK1 ELISA Kit, ALP ELISA Kit, MPI ELISA Kit, BLPI ELISA Kit, HUSI ELISA Kit, HUSI-I ELISA Kit, WAP4 ELISA Kit, WFDC4 ELISA Kit, Antileukoproteinase ELISA Kit, Seminal proteinase inhibitor ELISA Kit, Mucus proteinase inhibitor ELISA Kit, WAP four-disulfide core domain protein 4 ELISA Kit

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Human Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit

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UniProt

P03973

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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