Human SERPINI2 protein

SKU: orb392471

Description

Recombinant human SERPINI2 protein is produced by our mammalian expression system and the target gene encoding Ser19-Leu405 is expressed with a 6His tag at the C-terminus.

Research Area

Epigenetics

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	Human cells
Target	SERPINI2
Reactivity	Human
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Protein Sequence	SRCSAQKNTE FAVDLYQEVS LSHKDNIIFS PLGITLVLEM VQLGAKGKAQ QQIRQTLKQQ ETSAGEEFFV LKSFFSAISE KKQEFTFNLA NALYLQEGFT VKEQYLHGNK EFFQSAIKLV DFQDAKACAE MISTWVERKT DGKIKDMFSG EEFGPLTRLV LVNAIYFKGD WKQKFRKEDT QLINFTKKNG STVKIPMMKA LLRTKYGYFS ESSLNYQVLE LSYKGDEFSL IIILPAEGMD IEEVEKLITA QQILKWLSEM QEEEVEISLP RFKVEQKVDF KDVLYSLNIT EIFSGGCDLS GITDSSEVYV SQVTQKVFFE INEDGSEAAT STGIHIPVIM SLAQSQFIAN HPFLFIMKHN PTESILFMGR VTNPDTQEIK GRDLDSLVDH HHHHH

Storage & Handling

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Storage	Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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MEPI; PI14; Serpin I2; Myoepithelium-derived serine protease inhibitor; Pancpin; Pancreas-specific protein TSA2004; Peptidase inhibitor 14; PI-14

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Quick Database Links

Gene Symbol

SERPINI2

UniProt

O75830

NCBI Gene ID

5276

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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