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Human Serum Albumin Antibody
SKU: orb2652785
Description
Images & Validation
−Item 1 of 2
| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:50,000 - 1:200,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Human Serum Albumin |
| Purity | Anti-Human Serum Albumin Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using purified human serum albumin. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Analysis by SDS-PAGE was used to determine that this preparation is substantially free of aggregates and shows a banding pattern consistent with purified Rabbit IgG. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-human serum albumin antibody, rabbit anti-HSA antibody, Analbuminemia antibody, rabbit anti-serum albumin antibody, rabbit anti-albumin antibody
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Dot Blot of Rabbit IgG protein. Antigen: control Rabbit IgG protein. Load: 250 ng starting total load in a serial 1:1 dilution. Primary antibody: Rabbit IgG protein. Secondary antibody: Rabbit IgG Antibody Peroxidase Conjugated and Rabbit IgG Antibody DyLight™ 649 Conjugated secondary antibodies at 1:20000 for 45 min at RT. Block: orb348637 buffer overnight at 4°C.
Western Blot of human serum albumin. Lane 1: 1:400 of FBS. Lane 2: 1:1600 of FBS. Lane 3: 1:400 of normal rabbit serum. Lane 4: 1:1600 of normal rabbit serum. Lane 5: 1:400 of normal human serum. Lane 6: 1:1600 of normal human serum. Lane 7: 1:10 of human serum albumin (10 mg/ml). Lane 8: 1:40 of human serum albumin (10 mg/ml). Lane 9: molecular weight. Right Blot: Secondary only incubated for control. Primary antibody: human serum albumin at 1:400 for overnight at 4°C. Secondary antibody: Biotin conjugated rabbit anti human serum albumin with 10 mg/ml BSA added to stock was diluted 1:5000 in PBST for 2hrs at RT. DyLight 649 conjugated Streptavidin 1:50000 in PBST 1 hr RT was used as a secondary reagent. Block: 1.5% rabbit serum overnight at 4°C.
Quick Database Links
UniProt
RefSeq (Protein):NP_000468.1
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_000468.1 |
|---|
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot
Human Serum Albumin Antibody (orb2652785)
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