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Human Serum Albumin Antibody Peroxidase Conjugated
Description
Images & Validation
−| Tested Applications | ELISA, IHC, WB |
|---|---|
| Dilution range | ELISA: 1:10,000 - 1:50,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:5,000 |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Anti-Human Serum Albumin Antibody was produced by repeated immunizations with Human Serum Albumin. |
| Purity | Human Serum Albumin antibody was purified from pooled rabbit sera obtained from rabbits immunized with Human Serum Albumin. Purification was by Protein A Sepharose Fast Flow followed by POROS EP antigen resin affinity purification. The purified antibody was then conjugated to horseradish peroxidase and subsequently purified followed by exhaustive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum, purified human serum albumin IgG, and human Serum. |
| Conjugation | HRP |
Storage & Handling
−| Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/ml Polyethylene Glycol (PEG-8000); Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.0 mg/mL |
| Disclaimer | For research use only |
Alternative Names
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Western blot of Peroxidase conjugated Rabbit Anti-Human Serum Albumin secondary antibody. Lane 1: Human Serum Albumin (HSA). Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Peroxidase conjugated Rabbit Anti-Human Serum Albumin secondary antibody at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted\Observed size: 66 kDa, 61 kDa for HSA. Other band(s): None.
Quick Database Links
UniProt Details
−NCBI Reference Sequences
−| Protein | NP_000468.1 |
|---|
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Protocol Information
Human Serum Albumin Antibody Peroxidase Conjugated (orb2652782)
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