Human serum albumin

SKU: orb2563753

Description

Human Serum Albumin (HSA) is a key plasma protein that acts as a major endogenous inhibitor of amyloid-β aggregation, relevant to Alzheimer's disease research. It is widely utilized in vitro as a carrier protein in cell culture, drug delivery studies, and as a stabilizer in immunoassays and biopreservation.

Research Area

Immunology & Inflammation, Neuroscience, Signal Transduction

Images & Validation

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Key Properties

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CAS Number	70024-90-7
Target	Beta Amyloid,NO Synthase,NF-κB
Solubility	H2O:100 mg/mL

Bioactivity

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In Vivo

In a study on Male Swiss mouse models, Human serum albumin administered intravenously at a dose of 10 L/kg once at 1 hour and once at 5 hours effectively mitigate the oxidative stress and nitric oxide overproduction induced by lipopolysaccharide (LPS, 50 mg/kg via intraperitoneal injection) the treatment successfully inhibited iNOS expression and formation of peroxynitrite (ONOO−), reduce the upregulation of NF-κB, and prevente the decline in vascular response to phenylephrine, musclee tone, and endothelial function caused by endotoxin.

In Vitro

Human serum albumin (HSA) at a concentration of 5 μM for 0-90 minutes can bind with Glycerol monolaurate (GML), with a dissociation constant (K d) of 1.4 μM, and it serves to protect T cell function by preventing mL from inhibiting human T cells. HSA at concentration of 0.01, 0.05, and 0.1 μM over 0-24 hours can mitigat the GML-induced phosphorylation of AKT at threonine 308 and serine 473, as well a the formation of LAT, PLC-γ1, and AKT microclusters, while restorin the production of IFN-γ, IL-2, IL-10, and TNF-α in GML-treated cells. Western Blot analysis in activated peripheral blood T cells at a 0.05 μM concentration with incubation times of 0, 2, 5, and 15 minutes showed that it affected GML's ability to inhibit AKT phosphorylation. RT-PCR studies, using activated peripheral blood T cells with concentration of 0.1, 0.05, and 0.005 μM over 24 hours, demonstrate the restoration of IFN-γ, IL-2, IL-10, and TNF-α production in GML-treated cells.

Storage & Handling

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Storage	keep away from direct sunlight,keep away from moisture \| store at 4°C \| Shipping with blue ice.
Disclaimer	For research use only

Alternative Names

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HSA

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Gene Symbol

Beta Amyloid,NO Synthase,NF-κB

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