Human Sex Determining Region Y Box Protein 10 (SOX10) ELISA Kit

SKU: orb866906

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Description

Sandwich Human SOX10 ELISA Kit

Research Area

Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human SOX10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human SOX10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human SOX10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human SOX10 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.048 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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DOM ELISA Kit, MGC15649 ELISA Kit, SOX 10 ELISA Kit, SRY (sex determining region Y) box 10 ELISA Kit, SRY box 10 ELISA Kit, SRY box containing gene 10 ELISA Kit, SRY related HMG box gene 10 ELISA Kit

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Human Sex Determining Region Y Box Protein 10 (SOX10) ELISA Kit

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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