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Human TGFBR1 Protein, N-hFc Tag
SKU: orb689441
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Description
Research Area
Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 4
Key Properties
−| Source | Mammalian |
|---|---|
| Expression System | HEK293 |
| Target | TGFBR1 |
| Reactivity | Human |
| Tag | N-Human Fc Tag |
| Molecular Weight | The protein has a predicted molecular mass of 36.3 kDa after removal of the signal peptide.The apparent molecular mass of hFc-TGFBR1 is approximately 40-53 kDa due to glycosylation. |
| Expression Region | hFc(Glu99-Ala330) TGFBR1(Leu34-Leu126) |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Disclaimer | For research use only |
Alternative Names
−AAT5;ACVRLK4;ALK-5;ALK5;ESS1;LDS1;LDS1A;LDS2A;MSSE;SKR4;tbetaR-I;TBR-i;TBRI;TGFR-1
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Human CTLA-4 Protein, hFc Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human CTLA-4, hFc tagged protein (orb689463) can bind Anti-CTLA4 Neutralizing antibody orb689327 in a linear range of 0.64-80.0 ng/ml.
HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CTLA4, hFc tagged protein (orb689463) 1 μg/ml followed by Alexa 488-conjugated anti-human IgG secondary antibody.
Flow cytometry analysis with 1 μg/mL Human CTLA4 Protein, hFc tag (orb689463) on Expi293 cells transfected with human B7-1 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
Quick Database Links
Gene Symbol
TGFBR1
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
Human TGFBR1 Protein, N-hFc Tag (orb689441)
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