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Human TNFa Protein, His Tag
SKU: orb689423
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Description
Research Area
Epigenetics, NF-kB, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 4
Key Properties
−| Source | Mammalian |
|---|---|
| Expression System | HEK293 |
| Target | TNFA |
| Reactivity | Human |
| Tag | C-6×His Tag |
| Molecular Weight | The protein has a predicted molecular mass of 18.1 kDa after removal of the signal peptide. |
| Expression Region | TNFα(Arg78-Leu233) 6×His tag |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
|---|---|
| Form/Appearance | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Buffer/Preservatives | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−DIF;TNF-alpha;TNFA;TNFSF2;cachexin;cachectin;TNFα
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Human TNFα Protein, His Tag on SDS-PAGE under reducing condition.
ELISA plate pre-coated by 1 μg/ml (100 μL/well) Human TNFa, His tagged protein (orb689423) can bind Anti-TNFa Neutralizing antibody ([getskuurl sku"orb1173644"]) in a linear range of 0.64-16 ng/ml.
ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFα Protein, His Tag (orb689423) can bind Anti-TNFa (Certolizumab biosimilar) mAb (orb1826906) in a linear range of 0.64–16 ng/ml.
ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFα Protein, His Tag (orb689423) can bind Anti-TNFa (golimumab biosimilar) mAb (orb1826910) in a linear range of 3.20–80 ng/ml.
Quick Database Links
Gene Symbol
TNFA
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
Human TNFa Protein, His Tag (orb689423)
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