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Description
Research Area
Images & Validation
−| Application Notes |
|---|
Key Properties
−| Source | Mammalian cell |
|---|---|
| Biological Origin | Homo sapiens (Human) |
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human 4-1BBL-His at 10μg/ml can bind Human 4-1BB-Fc, the ED50 of Human 4-1BB-Fc is 16.8 ng/ml. |
| Tag | C-terminal Fc-tagged |
| Expression Region | 24-186aa |
| Protein Length | Extracellular Domain |
| Endotoxins | Less than 1.0 EU/μg as determined by LAL method. |
| MW | 44.2 kDa |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Protein Sequence | LQDPCSNCPAGTFCDNNRNQICSPCPPNSFSSAGGQRTCDICRQCKGVFRTRKECSSTSNAECDCTPGFHCLGAGCSMCEQDCKQGQELTKKGCKDCCFGTFNDQKRGICRPWTNCSLDGKSVLVNGTKERDVVCGPSPADLSPGASSVTPPAPAREPGHSPQ |
Storage & Handling
−| Storage | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃. |
|---|---|
| Form/Appearance | Lyophilized powder |
| Buffer/Preservatives | Lyophilized from a 0.2 μm filtered 1xPBS, pH 7.4 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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100 μg, 1 mgRecombinant Human 4-1BB Protein (Biotin) [orb2993093]
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SDS-PAGE: Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 19.9 KDa. Observed: 27-35 KDa, reducing conditions
20 μg, 100 μgRecombinant Human 4-1BB Protein [orb2994054]
Unconjugated
SDS-PAGE: Greater than 90% as determined by reducing SDS-PAGE.
Predicted: 43.9 KDa. Observed: 50-70 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mgRecombinant Human 4-1BB Protein [orb2994490]
Unconjugated
Greater than 95% as determined by reducing SDS-PAGE.
Predicted: 44 KDa. Observed: 55-75 KDa, reducing conditions
10 μg, 50 μg, 500 μg, 1 mg

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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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UniProt Details
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Protocol Information
Human TNFRSF9 protein (orb594869)
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