TPBG/5T4 Mouse Monoclonal Antibody

SKU: orb2974608

Description

Anti-Human TPBG/5T4 Antibody (BUCC2793) is a mouse monoclonal antibody detecting 5T4 in ELISA, FCM, SPR. Suitable for Human. Highlights Multi-Application: Validated across multiple applications. Flow Cytometry: Validated for flow cytometry applications.

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FC, SBR
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	BUCC2793
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Endotoxin	Please contact with the lab for this information.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M PBS, pH 7.4, 0.09% Sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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5T4, TPBG, WAIF1, M6P1, 5T4 oncofetal trophoblast glycoprotein, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Detects Recombinant Human TPBG/5T4 Protein, C-His (Cat No.: orb2966379) in indirect ELISA.

Flow-cytometry using anti-human TPBG antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human TPBG monoclonal antibody (Catalog: orb2974608) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human TPBG antibody. HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TPBG antibody monoclonal antibody (Catalog # orb2974608, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human TPBG antibody. MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TPBG antibody monoclonal antibody (Catalog # orb2974608, Green Histogram) at a concentration of 5 µg/mL for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody and cells analysed on a NovoCyte Flow Cytometer.

Quick Database Links

UniProt

Q13641

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No UniProt data available

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Protocol Information

Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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