Human UBA5 protein

SKU: orb392648

Description

Recombinant human UBA5 protein is produced by our E. coli expression system and the target gene encoding Met1-Met404 is expressed with a 6His tag at the N-terminus.

Research Area

Cell Biology

Images & Validation

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Tested Applications	ELISA, MS, SDS-PAGE, WB
Application Notes	Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Key Properties

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Source	E. coli
Target	UBA5
Reactivity	Human
Endotoxins	Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test.
Purity	Greater than 95% as determined by reducing SDS-PAGE.
Protein Sequence	MGSSHHHHHH SSGLVPRGSH MAESVERLQQ RVQELERELA QERSLQVPRS GDGGGGRVRI EKMSSEVVDS NPYSRLMALK RMGIVSDYEK IRTFAVAIVG VGGVGSVTAE MLTRCGIGKL LLFDYDKVEL ANMNRLFFQP HQAGLSKVQA AEHTLRNINP DVLFEVHNYN ITTVENFQHF MDRISNGGLE EGKPVDLVLS CVDNFEARMT INTACNELGQ TWMESGVSEN AVSGHIQLII PGESACFACA PPLVVAANID EKTLKREGVC AASLPTTMGV VAGILVQNVL KFLLNFGTVS FYLGYNAMQD FFPTMSMKPN PQCDDRNCRK QQEEYKKKVA ALPKQEVIQE EEEIIHEDNE WGIELVSEVS EEELKNFSGP VPDLPEGITV AYTIPKKQED SVTELTVEDS GESLEDLMAK MKNM

Storage & Handling

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Storage	Store it at -20°C to -80°C for one year. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.2 μM filtered solution of 20mM Tris-HCl, 1mM DTT, 10% glycerol, 50mM NaCl, pH 8.0.
Expiration Date	6 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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UBE1DC1; Ubiquitin-like modifier-activating enzyme 5; Ubiquitin-activating enzyme 5; ThiFP1; UFM1-activating enzyme; Ubiquitin-activating enzyme E1 domain-containing protein 1

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Quick Database Links

Gene Symbol

UBA5

UniProt

Q9GZZ9

NCBI Gene ID

79876

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Datasheet

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Protocol Information

Protein Handling and Storage Guide

Protein Handling Guide

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Western Blot (IB, immunoblot)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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SDS-PAGE

Sodium Dodecyl Sulphate PolyAcrylamide Gel Electrophoresis

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