Human WD Repeat Containing Protein 1 (WDR1) ELISA Kit

SKU: orb866885

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Description

Sandwich Human WDR1 ELISA Kit

Research Area

Signal Transduction

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human WDR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human WDR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human WDR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human WDR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	Tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.061 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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Actin interacting protein 1 ELISA Kit, Actin-interacting protein 1 ELISA Kit, AIP1 ELISA Kit, D5Wsu185e ELISA Kit, NORI 1 ELISA Kit, NORI-1 ELISA Kit, WD repeat domain 1 ELISA Kit, WD repeat-containing protein 1 ELISA Kit, wdr1 ELISA Kit, WDR1_HUMAN ELISA Kit

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Human WD Repeat Containing Protein 1 (WDR1) ELISA Kit

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UniProt

O75083

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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