Human Y-Box Binding Protein 3 (YBX3) ELISA Kit

SKU: orb866913

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Description

Sandwich Human YBX3 ELISA Kit

Research Area

Cancer Biology

Images & Validation

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Item 1 of 1

Tested Applications

ELISA

Application Notes

standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human YBX3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human YBX3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human YBX3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human YBX3 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

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Reactivity	Human
Sample Types	tissue homogenates, cell lysates and other biological fluids
Assay Type	Sandwich
Assay Time	3.5h
Range	0.16-10 ng/mL
Sensitivity	0.056 ng/mL
Concentration	10 ng/mL

Storage & Handling

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Storage	Refer to the Storage Guidelines in the Manual
Expiration Date	Please enquire.
Disclaimer	For research use only

Alternative Names

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BP8 ELISA Kit, CSDA2 ELISA Kit, CSDB ELISA Kit, DBPB ELISA Kit, MDR-NF1 ELISA Kit, NSEP1 ELISA Kit, YB1 ELISA Kit, Nuclease Sensitive Element Binding Protein 1 ELISA Kit, CCAAT-binding transcription factor I subunit A ELISA Kit, DNA-binding protein B ELISA Kit

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Human Y-Box Binding Protein 3 (YBX3) ELISA Kit

Quick Database Links

UniProt

P16989

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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