Hydroxyfasudil Hydrochloride

SKU: orb1302997

Description

Research Area

Cell Biology, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

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Key Properties

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CAS Number	155558-32-0
MW	343.83
Purity	99.82%
Formula	C₁₄H₁₈ClN₃O₃S
SMILES	Cl.O=c1[nH]ccc2c(cccc12)S(=O)(=O)N1CCCNCC1
Target	ROCK
Solubility	DMSO:62 mg/mL (180.32 mM)

Bioactivity

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Target IC50	PKA:37 μM\|ROCK1:0.73 μM\|ROCK2:0.72 μM
In Vivo	Hydroxyfasudil (10 mg/kg, i.p.) markedly increases both the maximal and average voided volumes and reduces maximal detrusor pressure in SD rats. In spontaneously hypertensive rats (SHRs), Hydroxyfasudil (3 mg/kg, i.p.) inhibits norepinephrine-induced hypercontractility and significantly ameliorates decreased penile cGMP contents at doses of 3/10 mg/kg, i.p. Read more...
In Vitro	Hydroxyfasudil has a weak inhibition of PKA (IC50: 37 μM), 50-fold higher than those of the ROCKs. Hydroxyfasudil increases eNOS mRNA levels (EC50: 0.8 ± 0.3 μM). Hydroxyfasudil (0-100 μM) concentration-dependently increases eNOS activity and stimulates NO production in HAEC. Hydroxyfasudil (10 μM) increases the half-life of eNOS mRNA from 13 to 16 hours but does not affect eNOS promoter activity (0.1-100 μM). Read more...
Animal Research	Hydroxyfasudil is formulated in saline.Micturition behavior is studied after administration of either Hydroxyfasudil (10 mg/kg, i.p.) or a corresponding volume of saline. Each rat is placed in a metabolic cage containing a urine collection funnel that is placed over an electronic balance. The balance is connected to a personal computer via a multiport controller and used to measure the cumulative weight of the collected urine. Every 150 s during a continuous 24-h period, the computer samples and records the data for the micturition frequency and volumes. The micturition reflex parameters that are collected include: urine volume per micturition, maximal micturition volume, micturition frequency, and total urine output in the Hydroxyfasudil- or vehicle-treated animals. Each monitoring session started at 18.00 hours. Prior to being placed in the metabolic cage at the start of each experimental period, the animals received either a single injection of Hydroxyfasudil (10 mg/kg) dissolved in saline or an injection of saline without the inhibitor. Read more...

Storage & Handling

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Storage	Powder: -20°C for 3 years \| In solvent: -80°C for 1 year \| Shipping with blue ice/Shipping at ambient temperature.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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inhibit, HA 1100, HA 1100 hydrochloride, HA 1100 Hydrochloride, HA1100, HA-1100, HA1100 Hydrochloride, HA-1100 Hydrochloride, Hydroxyfasudil, Hydroxyfasudil (HA-1100) HCl, Hydroxyfasudil Hydrochloride, Inhibitor, ROCK, ROK, Rho-kinase, Rho-associated protein kinase, Rho-associated kinase

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Compound Identifiers

PubChem CID

11371328 ↗

Key Properties

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No computed properties available.

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