You have no items in your shopping cart.
ICAM-1 Rabbit Polyclonal Antibody
SKU: orb1294365
Description
Research Area
Cardiovascular Research
Images & Validation
−Item 1 of 6
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | Western Blot 1:500, Immunofluorescence 1:200–1:500, Immunohistochemistry (Paraffin) 1:100-1:200 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / Extracellular domain corresponding to Human ICAM-1 / CD54 |
| Target | ICAM-1 / CD54 (Extracellular) |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Similar Products
−- Item 1 of 9
ICAM1 Rabbit Polyclonal Antibody [orb402187]
ELISA, FC, ICC, IF, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
100 μg - Item 1 of 4
ICAM1 Rabbit Polyclonal Antibody [orb10334]
IF, IHC-Fr, IHC-P, WB
Rat
Human
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl - Item 1 of 5
ICAM1 Rabbit Polyclonal Antibody [orb180715]
FC, ICC, IF, IHC, IHC-Fr, WB
Human
Rabbit
Polyclonal
Unconjugated
100 μg - Item 1 of 5
- Item 1 of 2
ICAM-1 (phospho Tyr512) rabbit pAb Antibody [orb764208]
ELISA, WB
Human, Mouse, Rat
Polyclonal
Unconjugated
100 μl, 50 μl
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
ICAM-1 / CD54 antibody - at 1/500 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294365 overnight at 4°C.
ICAM-1 / CD54 antibody - at 1/500 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294365 overnight at 4°C.
ICAM-1 / CD54 antibody - at 1/500 dilution, Lysates/proteins at 30 µg per lane. This blot was produced using a 7.5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294365 overnight at 4°C.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294365 anti-ICAM-1 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294365 anti-ICAM-1 antibody (red) at 1:100 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling ICAM-1 at 1/100.
Quick Database Links
Gene Symbol
ICAM-1 / CD54 (Extracellular)
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ICAM-1 Rabbit Polyclonal Antibody (orb1294365)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Usually dispatched within 5-10 working days