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Description
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| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:2000-1:10000, WB: 1:500-1:1000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | IFN Induced GTPase Like Protein was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal region of mouse IFN Induced GTPase Like Protein. |
| Target | Gm4841 |
| Purity | Anti-IFN Induced GTPase Like Protein Antibody was prepared from monospecific, delipidated and defibrinated antiserum, with addition of sodium azide to 0.01%. A BLAST analysis was used to suggest cross-reactivity with IFN Induced GTPase Like Protein from mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 80 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-IFN Induced GTPase-like Protein Antibody, interferon-gamma induced, Interferon-gamma-inducible GTPase Ifgga3 protein
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Western Blot of Rabbit Anti-IFN GTPase antibody. Lane 1: WT mouse spleen lysate. Lane 2: IFN gamma gene KO mouse spleen lysate. Load: 35 µg per lane. Primary antibody: IFN GTPases antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: [KO gene results in chronic and persistent IFN gamma protein in the sera] ~52kDa for IFN GTPases antibody. Other band(s): nonspecific.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001030031.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Gm4841 Antibody (orb750548)
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