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Description
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| Tested Applications | ELISA, WB |
|---|---|
| Dilution Range | ELISA: 1:2000 - 1:10,000, WB: 1:500-1:1000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | IFN Induced GTPase Like Protein was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal region of mouse IFN Induced GTPase Like Protein. |
| Target | Gm4841 |
| Purity | Anti-IFN Induced GTPase Like Protein Antibody was prepared from monospecific, delipidated and defibrinated antiserum, with addition of sodium azide to 0.01%. A BLAST analysis was used to suggest cross-reactivity with IFN Induced GTPase Like Protein from mouse based on a 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
| Concentration | 80 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Dry Ice Shipping | Please note: This product requires shipment on dry ice. A dry ice surcharge will apply. |
| Disclaimer | For research use only |
Alternative Names
−rabbit anti-IFN Induced GTPase-like Protein Antibody, interferon-gamma induced, Interferon-gamma-inducible GTPase Ifgga3 protein
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Western Blot of Rabbit Anti-IFN GTPase antibody. Lane 1: WT mouse spleen lysate. Lane 2: IFN gamma gene KO mouse spleen lysate. Load: 35 µg per lane. Primary antibody: IFN GTPases antibody at 1:1000 for overnight at 4°C. Secondary antibody: IRDye800™ rabbit secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: [KO gene results in chronic and persistent IFN gamma protein in the sera] ~52kDa for IFN GTPases antibody. Other band(s): nonspecific.
Quick Database Links
UniProt Details
− No UniProt data available
NCBI Reference Sequences
−Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product| Protein | NP_001030031.2 |
|---|
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Protocol Information
WB
Western Blot (IB, immunoblot)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Gm4841 Antibody (orb750549)
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