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IGF1 Antibody
SKU: orb1261109
Featured
Description
Images & Validation
−Item 1 of 7
| Tested Applications | IF, IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human IGF1 (NP_000609.1). |
| Target | IGF1 |
| Molecular Weight | Observed: 22kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Liquid |
| Concentration | batch dependent |
| Disclaimer | For research use only |
Alternative Names
−IGF1, OTTHUMP00000195084, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor 1, IGFI, IGF1A, IBP1
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Western blot analysis of extracts of various cell lines, using IGF1 antibody (orb1261109) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat lung using IGF1 antibody (orb1261109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using IGF1 antibody (orb1261109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using IGF1 antibody (orb1261109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using IGF1 antibody (orb1261109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using IGF1 antibody (orb1261109) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using IGF1 antibody (orb1261109) at dilution of 1:100 (40x lens).
Quick Database Links
Gene Symbol
IGF1
UniProt
UniProt Details
− No UniProt data available
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IGF1 Antibody (orb1261109)
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